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ACTU US Equity

Actuate Therapeutics, Inc.Health Care · Pharmaceutical Preparations · CIK 1652935 · FY ends Dec 31
$1.06
+0.01 (+0.95%)
USD · as of 2026-08-19 · marketstack

ACTU · 10-K · period ended 2024-12-31

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filed 2025-03-13 · EDGAR original ↗

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Item 7. Management’s Discussion and Analysis of Financial Condition

and Results of Operations.

The following discussion and

analysis of the financial condition and results of our operations should be read together with the consolidated financial statements and

related notes of Actuate Therapeutics, Inc. included in Part II Item 8 of this Annual Report on Form 10-K (“Report”).

This discussion and analysis

contains forward-looking statements reflecting our management’s current expectations that involve risks, uncertainties and assumptions.

See the section entitled “Cautionary Note Regarding Forward-Looking Statements.” Our actual results and the timing of events

may differ materially from those described in or implied by these forward-looking statements due to a number of factors, including those

discussed below and elsewhere in this Report, particularly those set forth under “Risk Factors.”

Business Overview

We are a clinical stage biopharmaceutical

company focused on developing therapies for the treatment of high impact, difficult to treat cancers through the inhibition of glycogen

synthase kinase-3 (“GSK-3”). We are developing elraglusib (formerly 9-ING-41), an ATP-competitive small molecule that is designed

to enter cancer cells and block the function of the enzyme glycogen synthase kinase-3 beta (“GSK-3β”), a master regulator

of complex biological signaling cascades, including those mediated by oncogenes, that lead to tumor cell survival, growth, migration,

and invasion. We believe that the blockade of GSK-3β signaling ultimately results in the death of the cancer cells and the regulation

of anti-tumor immunity.

We have exclusively licensed a

portfolio of GSK-3 inhibitors developed in a collaboration between The Board of Trustees of the University of Illinois-Chicago (“UIC”)

and Northwestern University (“NU”). Elraglusib is the lead investigational product in our portfolio and is being evaluated

in a Phase 2 trial in patients with metastatic pancreatic ductal adenocarcinoma (“mPDAC”), our most advanced clinical indication

to date. We are also advancing a Phase 1/2 clinical trial in refractory pediatric malignancies, including Ewing sarcoma (“EWS”).

Elraglusib represents a broad

opportunity for us to potentially initiate and advance multiple drug development programs around our lead asset based on data emerging

from completed or ongoing Phase 1/2 trials and non-clinical biological, cellular, and animal data. Animal tumor model data, Phase 1/2

clinical data and AI-based computational approaches have identified a number of areas of unmet clinical need in cancer where elraglusib

may play an interventional role, including pancreatic, metastatic melanoma, lung, colon, breast, renal, and ovarian cancer, leukemias

and lymphomas, as well as some pediatric cancers including Ewing sarcoma, neuroblastoma and pediatric leukemias.

Our lead clinical program, referred

to as Actuate-1801, is an intravenous (“IV”) injection solution of elraglusib (“Elraglusib Injection”) that we

are evaluating for the treatment of first-line mPDAC. In addition, Elraglusib Injection is also being evaluated in a Phase 1/2 clinical

trial in refractory pediatric malignancies and the data from this study (Actuate-1902) identified Ewing sarcoma as a potential second

indication for further development of Elraglusib Injection.

We have developed several oral

dosage forms of elraglusib, which we believe will allow us to expand the number of cancer indications that we are able to target and allow

us to further explore more convenient dose delivery options for patients. A clinical candidate tablet, the Elraglusib Oral Tablet, has

been selected for further development and we are planning a Phase 1 study (Actuate-2401) to identify the maximum tolerated dose (“MTD”)

and recommended Phase 2 dose (“RP2D”) for Elraglusib Oral Tablet in patients with advanced, refractory adult cancers subject

to future funding. Subject to additional funding, several Phase 2 studies have been identified for further clinical development of Elraglusib

Oral Tablet based on data from the Actuate-1801 study in indications, including but not limited to, refractory, metastatic melanoma and

refractory, metastatic colorectal cancer.

Since our inception in 2015, we

have focused substantially all of our resources on organizing and staffing our Company, business planning, raising capital, establishing

and maintaining our intellectual property portfolio, conducting research, preclinical studies, and clinical trials, establishing arrangements

with third parties for the manufacture of elraglusib, and providing general and administrative support for these operations. We do not

have any products approved for sale and have not generated any revenue from product sales since inception.

We have incurred significant operating

losses and negative cash flows from operations since our inception. Our net losses were $27,285,328 and $24,744,620 for the years ended

December 31, 2024 and 2023, respectively. As of December 31, 2024, we had an accumulated deficit of $132,379,849. Substantially all of

our net losses have resulted from costs incurred in connection with our research and development programs and, to a lesser extent, from

general and administrative costs associated with our operations. We expect to continue to incur significant expenses and operating losses

in the foreseeable future, and we anticipate these losses will increase substantially as we continue our development of, seek regulatory

approval for, and potentially commercialize elraglusib, and potentially seek to discover and develop additional product candidates, utilize

third parties to manufacture elraglusib, hire additional personnel, expand and protect our intellectual property, and incur additional

costs associated with being a public company. If we obtain regulatory approval for elraglusib, we expect to incur significant expenses

related to developing our commercialization capability to support product sales, marketing and distribution.

Because of the numerous risks

and uncertainties associated with pharmaceutical product development, we are unable to accurately predict the timing or amount of increased

expenses or when, or if, we will be able to achieve or maintain profitability. Even if we are able to generate product sales, we may not

become profitable. If we do not become profitable or are unable to sustain profitability on a continuing basis, then we may be unable

to continue our operations at planned levels and may be forced to reduce or terminate our operations.

As of December 31, 2024, we had

cash and cash equivalents of $8,641,622. Based on our current operating plan, we estimate that our existing cash and cash equivalents

as of the date of this Report will not satisfy the Company’s operational and capital requirements through twelve months from the

issuance date of the consolidated financial statements included in this Report.

Also, we will not generate revenue

from product sales unless and until we successfully complete clinical development and obtain regulatory approval for elraglusib or any

future product candidates, which we expect will take a number of years and may never occur. As a result, we will need substantial additional

funding to support our continuing operations and pursue our business strategy. Until such time we can generate significant revenue from

product sales, if ever, we expect to finance our operations through equity offerings, debt financings, or other capital sources, including

potential future collaborations, licenses, and other similar arrangements. As we seek additional financing in the near term, we may be

unable to raise additional funds or enter into such other agreements or arrangements when needed on favorable terms, or at all. If we

fail to raise capital or enter into such agreements or arrangements as, and when needed, we may delay, limit, reduce or terminate our

product development or future commercialization efforts or grant rights to develop and market product candidates that we would otherwise

prefer to develop and market ourselves, or even cease operations.

Recent Developments

IPO

On August 14, 2024, the Company

completed the closing of its IPO of 2,800,000 shares of common stock at an initial offering price to the public of $8.00 per share, before

the underwriters discount of $0.56 per share. Additionally, the underwriters exercised their option (“Overallotment Option”)

to purchase an additional 420,000 shares at the same price of $8.00 per share less the underwriters discount on September 12, 2024. The

Company’s common shares began trading on the Nasdaq Global Market on August 13, 2024, under the symbol "ACTU". The Company

received net proceeds of approximately $22 million, after deducting discounts and commissions and other offering expenses of approximately

$3.7 million for the issuance of 3,220,000 shares of common stock of the Company, including shares issued under the Overallotment Option.

Upon the closing of the IPO and

Overallotment Option, we issued the underwriters warrants (“Underwriter Warrants”) to purchase up to 161,000 shares of common

stock, representing 5% of the shares of common stock issued under the IPO and Overallotment Option, at an exercise price of $10.00 per

share, representing 125% of the initial offering price. The Underwriter Warrants are not exercisable prior to February 8, 2025 (or 180-days

from the effective date of the registration statement), and expire on August 12, 2027. The Underwriter Warrants can only be exercised

on a cash basis through November 11, 2025 and only on a cashless basis on November 12, 2025 and thereafter.

In addition, the Company’s

Redeemable Convertible Preferred Stock, Related Party Convertible Notes Payable and in-the-money warrants to purchase the Company’s

Redeemable Convertible Preferred Stock converted into or were automatically exercised for, as applicable, common stock immediately prior

to the closing of the IPO.

Authorized Capital

Effective upon the closing of

the Company’s IPO, the Company’s authorized capital consists of 200,000,000 shares of common stock, $0.000001 par value per share,

and 10,000,000 shares of preferred stock, $0.000001 par value per share.

Reverse Stock Split

On May 31, 2024, the Company’s

board of directors approved a 1-for-1.8 reverse stock split of its issued and outstanding shares of common stock and stock option awards,

which was effected on June 7, 2024. All issued and outstanding shares of common stock (including outstanding RSAs), stock option awards

and per share data have been adjusted in these consolidated financial statements, on a retrospective basis, to reflect the reverse stock

split for all periods presented.

Components of Our Results of Operations

Our operating expenses consist

of (i) research and development expenses and (ii) general and administrative expenses.

Research and Development Expenses

Research and development expenses

consist primarily of external and internal costs incurred in performing clinical and preclinical development activities. Our external

research and development costs primarily consists of the cost incurred under agreements with hospitals to treat and monitor patients enrolled

in our clinical trials, contract research organizations and contract manufacturers, consultants and other third parties to conduct and

support our clinical trials and preclinical studies. Our internal research and development costs primarily include research and development

personnel-related expenses such as employee compensation, employer taxes, group insurance benefits, and stock-based compensation.

We expense research and development

costs as incurred. We currently only have one product candidate, elraglusib. Therefore, since our inception, substantially all of our

research and development costs were related to the development of elraglusib. We track research and development expenses on an aggregate

basis and not on an indication-by-indication or treatment setting-by-treatment setting basis.

Although research and development

activities are central to our business model, the successful development of elraglusib and any future product candidates is highly uncertain.

There are numerous factors associated with the successful development of any product candidate such as elraglusib, including future trial

design and various regulatory requirements, many of which cannot be determined with accuracy at this time based on our stage of development.

In addition, future regulatory factors beyond our control may impact our clinical development programs. Product candidates in later stages

of clinical development generally have higher development costs than those in earlier stages of clinical development, primarily due to

the increased number of patients and duration of later-stage clinical trials. As a result, we expect our research and development expenses

will increase substantially in connection with our ongoing and planned clinical and preclinical development activities in the near term

and in the future, provided we are able to raise additional capital. At this time, we cannot accurately estimate or know the nature, timing

and costs of the efforts that will be necessary to complete the preclinical and clinical development of elraglusib and any future product

candidates. Our future research and development expenses may vary significantly based on a wide variety of factors such as:

· per patient trial costs;

· the number of trials required for approval;

· the number of doses that patients receive;

· the potential additional safety monitoring requested by regulatory agencies;

· the duration of patient participation in the trials and follow-up;

· the extent of changes in government regulation and regulatory guidance;

A change in the outcome of any

of these variables with respect to the development of elraglusib or any future product candidates could significantly change the costs

and timing associated with the development of that product candidate. We may never succeed in obtaining regulatory approval for any product

candidate.

General and Administrative Expenses

General and administrative expenses

consist primarily of personnel-related expenses such as employee compensation, benefits, and stock-based compensation, for our personnel

in executive and other administrative functions. General and administrative expenses also include legal fees relating to patent and corporate

matters and professional fees paid for accounting, auditing, consulting and tax services, as well as other costs such as insurance costs,

investor and public relations, and travel expenses.

We anticipate our general and

administrative expenses will increase substantially in the future as we expand our operations, including increasing our headcount to support

our continued research and development activities and preparing for later-stage clinical trials and potential commercialization of elraglusib.

We also anticipate we will continue to incur increased accounting, audit, legal, regulatory, compliance, director and officer insurance,

and investor and public relations expenses associated with operating as a public company.

Other Income (Expense)

Change in Fair Value of Warrant Liability

On June 30, 2023, in connection

with the issuance of the Series C Redeemable Convertible Preferred Stock, we issued the placement agent warrants to purchase up to 18,223

shares of Series C Redeemable Convertible Preferred Stock (after giving effect to the conversion of such shares into common stock) at

an exercise price equivalent to $9.42 per share of common stock. The initial estimated fair value of these warrants of $93,863 was calculated

using the Black-Scholes valuation model and recorded as a reduction to Redeemable Convertible Preferred Stock and a corresponding increase

in the warrant liability.

In 2018, in connection with convertible

promissory note payable agreements, we issued the noteholders warrants to purchase shares of Series B-1 Redeemable Convertible Preferred

Stock, of which, warrants to purchase up to 76,376 shares of Series B Redeemable Convertible Preferred Stock (after giving effect to the

conversion of such shares into common stock) were issued at an exercise price equivalent to $5.27 per share of common stock and warrants

to purchase up to 76,376 shares of Series B Redeemable Convertible Preferred Stock (after giving effect to the conversion of such shares

into common stock) were issued at an exercise price equivalent to $10.55 per share of common stock.

The Redeemable Convertible Preferred

Stock Warrants required liability classification as the underlying Redeemable Convertible Preferred Stock was considered contingently

redeemable and could have obligated us to transfer assets to the holders at a future date upon occurrence of a deemed liquidation event.

The warrants were recorded at fair value upon issuance and were subject to remeasurement to fair value at each balance sheet date, with

any changes in fair value recognized in other income (expense), net. We adjusted the warrant liability for changes in fair value until

the earlier of the exercise, conversion, or expiration of the Redeemable Convertible Preferred Stock Warrants. In July 2024, the Redeemable

Convertible Preferred Stock Warrants were amended to provide that if underlying Redeemable Convertible Preferred Stock Warrants were out-of-the-money

based on the initial public offering price in the IPO, the out-of-the-money Redeemable Convertible Preferred Stock Warrants would convert

into warrants to purchase common stock. Accordingly, the Redeemable Convertible Preferred Stock Warrants were remeasured upon the closing

of the IPO and marked to market to its fair value before being reclassified to equity.

Loss on Issuance of Related Party Convertible Notes

Payable; Change in Estimated Fair Value of Related Party Convertible Notes Payable

On February 20, 2024, March 27,

2024, and May 8, 2024, the Company issued related party convertible notes in the amount of $3,000,000, $1,500,000, and $1,000,000 (collectively,

“Related Party Convertible Notes Payable”), respectively. The Related Party Convertible Notes Payable were measured at fair

value on their issuance date and remeasured at estimated fair value at the end of each reporting period with changes in fair value recognized

as a component of other income (expense). Upon issuance of the Related Party Convertible Notes Payable, we elected to apply the fair value

option to the Related Party Convertible Notes Payable in accordance with Accounting Standards Codification (“ASC”) 825, Financial

Instruments. In certain circumstances, the estimated fair value at issuance may be greater than the principal amount at issuance.

The loss on issuance of the Related Party Convertible Notes Payable represented the difference between the estimated fair value of the

Related Party Convertible Notes Payable on the issuance date and the gross proceeds received on the issuance date based on the valuation

assumptions, including but not limited to, the proximity in time to the IPO, the discount on conversion of the Related Party Convertible

Notes Payable upon a financing or IPO, and the increased probability weighted IPO scenario on the issuance date.

Prior to the closing of the Company’s

IPO, the fair value of the Related Party Convertible Notes Payable was estimated at each reporting period using a scenario-weighted binomial

lattice model to calculate equity values at different points in time leading up to a conversion event. Assumptions in the model included

but were not limited to the following: equity value, conversion price, accrued interest, volatility, risk-free interest rate, dividend

yield, time to a conversion event, and scenario weightings. Accrued interest on the Related Party Convertible Notes Payable was included

in the determination of the estimated fair value.

In connection with the closing

of the Company’s IPO on August 14, 2024, the Company issued Bios Clinical Opportunity Fund, LP 884,427 shares of its common stock

upon the conversion of the Related Party Convertible Notes Payable, including accrued interest thereon, at a conversion price of $6.40

per share, representing 80% of the IPO price of $8.00 per share. The Related Party Convertible Notes Payable was marked to market to its

fair value on the conversion date before being reclassified to equity. The aggregate fair value at the time of conversion was calculated

by multiplying the number of shares of common stock issued upon conversion by the fair value per share on the conversion date, which was

the closing price of the Company’s common stock on the Nasdaq Global Market on the closing date of the IPO.

Interest Expense

Interest expense represents interest

owed to UIC under our license agreement with UIC, whereby UIC agreed to defer amounts owed to UIC under a former sublicense agreement

in the amount of $404,991.

Interest Income

Interest income represents interest

earned on our cash and cash equivalents at the then prevailing market rates.

Results of Operations

Comparison of the Year Ended December 31, 2024 and 2023:

The following table summarizes our results of operations

for the year ended December 31, 2024 and 2023:

Year Ended December 31,

Operating expenses:

Other income (expense):

Research and Development Expenses

The following table summarizes our research and development

expenses for the year ended December 31, 2024 and 2023:

Year Ended December 31,

The decrease in research and development

expenses of $3,032,056 for the year ended December 31, 2024 compared to the prior year was primarily due to (i) a decrease in preclinical

and biomarker studies in the current period of $1,402,916 due to few contracted studies in the current year, (ii) a decrease in external

clinical trial expenses of $573,612 mostly related to lower contract research organizations (“CRO”) costs in the current year

related to the randomized Phase 2 mPDAC trial (Actuate-1801 Part 3B) associated with fewer patients on study, (iii) a decrease of $539,148

in Chemistry Manufacturing & Control (“CMC”) related costs due to a decrease in manufacturing costs of elraglusib in the

current period due to the timing of drug substance manufacturing to support the randomized Phase 2 mPDAC trial (Actuate-1801 Part 3B),

and (iv) a decrease in personnel and consulting expenses of $516,380 primarily due to a decrease in consulting fees in the current period

as certain consultants transitioned to full-time employment at an overall lower cost to the Company.

General and Administrative Expenses

The following

table summarizes our general and administrative expenses for the year ended December 31, 2024 and 2023:

Year Ended December 31,

The increase in general and administrative

expenses of $3,218,961 for the year ended December 31, 2024 compared to the prior year was primarily due to (i) an increase in personnel-related

expenses of $1,943,643 primarily due to an increase in non-cash stock-based compensation expense of approximately $1.6 million related

to awards granted to the president and chief executive officer, the chief financial officer, members of the board of directors in connection

with the IPO, and other administrative award grants combined with an increase in payroll and bonus expense of approximately $0.3 million

mostly related to the hiring of the Company’s chief financial officer in connection with the IPO (ii) an increase in professional

and consulting fees of $774,006 primarily related to an increase in search firm related fees to identify and add three new board members

to the Company’s Board of Directors to comply with Nasdaq listing requirements, an increase in valuation services to support the

estimated fair market value of the Company’s common stock and other financial instruments, including the fair value of the Related

Party Convertible Notes Payable, an increase in legal fees related to additional corporate matters and intellectual property costs, and

an increase in audit and audit related fees associated with the annual and quarterly review of the Company’s financial statements,

and (iii) an increase in other expenses of $501,312 primarily due to an increase in the cost of directors and officer insurance during

the current period, combined with an increase in board fees, investor relations fees, and other public company expenses.

Other Income (Expense)

Other income (expense), net, for

the year ended December 31, 2024 and 2023 is comprised of the following:

Liquidity and Capital Resources

Sources of Liquidity

Since our inception, we have not

generated any revenue from product sales and have incurred significant operating losses and negative cash flows from operations. We expect

to incur significant expenses and operating losses in the foreseeable future as we advance the clinical development of elraglusib and

any future product candidates. As of December 31, 2024, we had cash and cash equivalents of $8,641,622.

Future Funding Requirements

We expect our expenses to increase

substantially in connection with our ongoing activities, particularly as we continue our development of, seek regulatory approval for,

and potentially commercialize elraglusib and potentially seek to discover and develop and/or license or acquire additional product candidates,

conduct our ongoing and planned clinical trials and preclinical studies, continue our research and development activities, utilize third

parties to manufacture elraglusib, hire additional personnel, expand and protect our intellectual property, and incur additional costs

associated with being a public company.

Cash used to fund our operating

expenses is impacted by the timing of when we pay these expenses, as reflected in the change in our outstanding prepaid expenses, accounts

payable, and other accrued expenses. The timing and amount of our funding requirements will depend on many factors, including:

Based on our current operating

plan, we estimate that our existing cash and cash equivalents as of the date of this Report will not satisfy the Company’s operational

and capital requirements through twelve months from the issuance date of the consolidated financial statements included in this Report.

We have no other committed sources

of capital. Until such time, if ever, as we can generate substantial product revenue, we expect to finance our operations through equity

offerings, debt financings, or other capital sources, including current or potential future collaborations, licenses, and other similar

arrangements. As we seek additional financing in the near future, we may be unable to raise additional funds or enter into such other

arrangements when needed on favorable terms or at all. To the extent we raise additional capital through the sale of equity or convertible

debt securities, stockholders’ ownership interest in our common stock will be diluted, and the terms of these securities may include

liquidation or other preferences that adversely affect the rights of our common stockholders. Debt financing and preferred equity financing,

if available, may involve agreements that include covenants limiting or restricting our ability to take specific actions, such as incurring

additional debt, making acquisitions, engaging in acquisition, merger or collaboration transactions, selling or licensing our assets,

making capital expenditures, redeeming our stock, making certain investments or declaring dividends. If we raise additional funds through

collaborations or license agreements with third parties, we may have to relinquish valuable rights to our technologies, future revenue

streams, research programs or product candidates, or grant licenses on terms that may not be favorable to us. If we are unable to raise

additional funds through equity, debt, or other financings when needed, we may be required to delay, limit, reduce or terminate our product

development or future commercialization efforts or grant rights to develop and market product candidates that we would otherwise prefer

to develop and market ourselves, or even cease operations.

Material Cash Requirements for Known Contractual and Other Obligations

Research and Development Costs

We are continuing to invest in

our elraglusib clinical trials and have entered into contractual obligations with each clinical trial site. Each contract shall continue

until the completion of the trial at that site. Our clinical trial costs are dependent on, among other things, the size, number and length

of our clinical trials.

Other Capital Requirements and Additional Royalty Obligations.

We enter into agreements in the

normal course of business with various vendors, which are generally cancellable upon notice. Payments due upon cancellation typically

consist only of payments for services provided or expenses incurred, including non-cancellable obligations of service providers, up to

the date of cancellation.

Cash Flow Summary

The following table provides a summary of our cash

flows for the year ended December 31, 2024 and 2023:

Year Ended December 31,

Cash Flows From Operating Activities

Year Ended December 31, 2024 — Net

cash used in operating activities for the year ended December 31, 2024 consisted of our net loss of $27,285,328 combined with the non-cash

gain on settlement of the warrant liability of $343,240, which amounts were offset by (i) non-cash stock-based compensation expense of

$1,995,793, (ii) a non-cash increase in the fair value of our warrant liability of $78,903, (iii) a loss on issuance of Related Party

Convertible Notes Payable at fair value of $400,000, (iv) the change in estimated fair value of Related Party Convertible Notes Payable

of $2,192,507, (v) an increase in accrued interest on license payable of $18,641, and (vi) cash provided by a net change in operating

assets and liabilities of $1,100,076.

Year Ended December 31,

2023 — Net cash used in operating activities for the year ended December 31, 2023 consisted of our net loss

of $24,744,620, which amount was offset by (i) non-cash stock-based compensation expense of $423,539, (ii) a non-cash increase

in the fair value of our warrant liability of $79,822, (iii) an increase in accrued interest on license payable of $43,641, and (iv) cash

provided by a net change in operating assets and liabilities of $2,572,451.

Cash Flows From Financing Activities

Year Ended December 31, 2024 — During

the year ended December 31, 2024, net cash provided by financing activities primarily consisted of net proceeds received from the closing

of the IPO and Overallotment Option of $22,025,611 (net of underwriting discounts and commissions and after payment of offering costs

of $1,931,189), proceeds of $5,500,000 from the issuance of the Related Party Convertible Notes Payable, which amount was offset .

Year Ended December 31, 2023 — During

the year ended December 31, 2023, net cash provided by financing activities consisted of net proceeds of $4,134,516 related to the issuance

of Series C Redeemable Convertible Preferred Stock.

Critical Accounting Policies and Significant Judgments and Estimates

Our financial statements are prepared

in accordance with accounting principles generally accepted in the United States of America (“U.S. GAAP”). The preparation

of our financial statements and related disclosures requires us to make estimates and judgments that affect the reported amounts of assets,

liabilities, costs and expenses, and the disclosure of contingent assets and liabilities in our financial statements. We base our estimates

on historical experience, known trends and events, and various other factors we believe are reasonable under the circumstances, the results

of which form the basis for making judgments about the carrying values of assets and liabilities that are not readily apparent from other

sources. We evaluate our estimates and assumptions on an ongoing basis. Our actual results may differ from these estimates under different

assumptions or conditions.

While our significant accounting

policies are described in more detail in Note 2 to the accompanying consolidated financial statements included elsewhere in this Report,

we believe the following accounting policies are those most critical to the judgments and estimates used in the preparation of our financial

statements.

Research and Development Expenses and Related Accrued Expenses

In accordance with authoritative

guidance, the Company charges research and development costs to operations as incurred. Research and development expenses consist primarily

of personnel and related costs, external costs of outside vendors engaged clinical trials, contract manufacturers, consultants and other

third parties to conduct and support our clinical trials and preclinical studies.

As part of the process of preparing

our consolidated financial statements, we are required to estimate our research and development expenses as of each balance sheet date.

This process involves reviewing open contracts, including clinical site contracts, and communicating with our personnel to identify services

that have been performed on our behalf, and estimating the level of service performed and the associated cost incurred for the service

when we have not yet been invoiced or otherwise notified of the actual cost. We make estimates of our research and development expenses

as of each balance sheet date based on facts and circumstances known to us at that time. The significant estimates in our research and

development expenses include the costs incurred for services performed by our vendors in connection with services for which we have not

yet been invoiced. We base our expenses related to research and development activities on our estimates of the services received and efforts

expended pursuant to quotes and contracts with contractors and vendors that conduct research and development on our behalf. The financial

terms of these agreements are subject to negotiation, vary from contract to contract, and may result in uneven payment flows. Advance

payments for goods and services that will be used in future research and development activities are expensed when the activity has been

performed or when the goods have been received rather than when the payment is made. Although we do not expect our estimates to be materially

different from amounts actually incurred, if our estimates of the status and timing of services performed differ from the actual status

and timing of services performed, it could result in us reporting amounts that are too high or too low in any particular period. To date,

there have been no material differences between our estimates of such expenses and the amounts actually incurred.

Stock-Based Compensation

In April 2015 and August 2024,

the Company’s Board of Directors (“Board”) adopted the 2015 Stock Incentive Plan (“2015 Plan”) and the 2024

Stock Incentive Plan (“2024 Plan”), respectively.

The Company periodically grants

equity-based payment awards in the form of restricted common stock awards (“RSAs”), restricted stock units (“RSUs”),

and stock options to employees, directors, consultants and non-employees and records stock-based compensation expenses for awards of stock-based

payments based on their estimated fair value at the grant date.

The estimated fair value of service-based

RSAs and RSAs are measured at the grant date based on the estimated fair market value of the Company’s common stock on the date

of grant and is recognized as expense over the requisite service period, which is generally the awards’ vesting period. The estimated

fair value of performance-based RSAs is measured at the grant date based on the estimated fair value of shares expected to be earned at

the end of the performance period, and is recognized as expense ratably over the performance period based upon the probable number of

shares expected to vest.

The Company accounts for the grant

of stock options based on the estimated fair value of the underlying option using the Black-Scholes valuation model on the date of grant

and are recognized as expense in the consolidated statement of operations on a straight-line basis over the requisite service period,

which is the vesting period. The Black-Scholes valuation model requires the input of subjective assumptions, including expected volatility,

expected dividend yield, expected term, risk-free rate of return and the estimated fair value of the underlying common stock on the date

of grant. Prior to the IPO, the Company regularly engaged a third-party valuation specialist to assist with estimates related to the valuation

of the Company’s common stock. Post IPO, the fair value of our common stock is determined based on the closing price of our common

stock as reported on the date of grant on the primary stock exchange on which our common stock is traded.

The Company classifies stock-based

compensation expense in the consolidated statements of operations in the same manner in which the award recipients’ payroll costs

are classified or in which the award recipients’ service payments are classified.

The Company recognizes forfeitures

related to stock-based compensation awards as they occur.

Determination of Fair Value of Our Common Stock

Prior to the closing of the Company’s

IPO, there was no public market for our common stock. Therefore, the estimated fair value of our common stock prior to the date of the

Company’s IPO was determined by our board of directors as of the date of grant of each award, with input from management, considering

our most recently available third-party valuations of common stock and our board of directors’ assessment of additional objective

and subjective factors that it believed were relevant and which may have changed from the date of the most recent valuation through the

date of the grant. These third-party valuations were performed in accordance with the guidance outlined in the American Institute of Certified

Public Accountants’ Accounting and Valuation Guide, Valuation of Privately-Held-Company Equity Securities Issued as Compensation.

Our common stock valuations were prepared using either an option pricing method (OPM) or a hybrid method, both of which used market approaches

to estimate our enterprise value. The OPM treats common stock and preferred stock as call options on the total equity value of a company,

with exercise prices based on the value thresholds at which the allocation among the various holders of a company’s securities changes.

Under this method, the common stock has value only if the funds available for distribution to stockholders exceed the value of the preferred

stock liquidation preferences at the time of the liquidity event, such as a strategic sale or a merger. The hybrid method is a probability-weighted

expected return method (PWERM) where the equity value in one or more of the scenarios is calculated using an OPM. The PWERM is a scenario-based

methodology that estimates the fair value of common stock based upon an analysis of future values for us, assuming various outcomes. In

addition to considering the results of these third-party valuations, our board of directors considered various objective and subjective

factors to determine the fair value of our common stock as of each grant date, including:

· our stage of development and business strategy;

The assumptions underlying these

valuations represented management’s best estimate, which involved inherent uncertainties and the application of management’s

judgment. As a result, if we had used significantly different assumptions or estimates, the fair value of our common stock and our stock-based

compensation expense could have been materially different.

There were significant judgments

and estimates inherent in the determination of the fair value of our common stock. Historically, these judgments and estimates included

assumptions regarding our future operating performance, the time to complete an IPO or other liquidity event, and the determination of

the appropriate valuation methods.

Based on our early stage of development,

the difficulty in predicting the range of specific outcomes (and their likelihood), and other relevant factors, the market approach was

considered most appropriate for valuations prior to the closing of the IPO. The recent transactions method was utilized to determine the

value of the equity and the OPM allocated the equity value to the respective share classes. In determining the estimated fair value of

our common stock, our board of directors also considered the fact that our stockholders could not freely trade our common stock in the

public markets prior to the closing of the Company’s IPO. Accordingly, we applied discounts to reflect the lack of marketability

of our common stock based on the weighted-average expected time to liquidity.

Once a public trading market for

our common stock became established in connection with the completion of the Company’s IPO, it is no longer necessary for our board

of directors to estimate the fair value of our common stock in connection with our accounting for granted equity-based awards or for any

other such awards we may grant, as the fair value of our common stock is determined based on the closing price of our common stock as

reported on the date of grant on the primary stock exchange on which our common stock is traded.

Fair Value of Financial Instruments

Authoritative guidance requires

disclosure of the fair value of financial instruments. The Company applies fair value measurements to record fair value adjustments to

certain assets and liabilities and to determine fair value disclosures. The carrying amount of certain of the Company’s financial

instruments, including cash and cash equivalents, accounts payable and accrued liabilities, approximate their estimated fair values primarily

due to the short-term nature of the instruments or based on information obtained from market sources and management estimates. The Related

Party Convertible Notes Payable and the Redeemable Convertible Preferred Stock Warrant Liability were carried at fair value until the

closing of the IPO based on unobservable market inputs. The Company measures the fair value of certain of its financial liabilities on

a recurring basis. A fair value hierarchy is used to rank the quality and reliability of the information used to determine fair values.

Financial assets and liabilities

carried at fair value which is not equivalent to cost will be classified and disclosed in one of the following three categories:

The Company reviews the fair value

hierarchy classification at each reporting date. Changes in the ability to observe valuation inputs may result in a reclassification of

levels for certain assets or liabilities within the fair value hierarchy. The Company did not have any transfers of assets and liabilities

between the levels of the fair value measurement hierarchy during the years presented.

Redeemable Convertible Preferred Stock Warrants

The Company’s Redeemable

Convertible Preferred Stock Warrants required liability classification and accounting as the underlying Redeemable Convertible Preferred

Stock was considered contingently redeemable and could have obligated the Company to transfer assets to the holders at a future date upon

occurrence of a deemed liquidation event. The warrants were recorded at their estimated fair value upon issuance and were subject to remeasurement

to estimated fair value at each balance sheet date, with changes in the estimated fair value recognized as a component of other income

(expense) in the accompanying consolidated statements of operations. The Company adjusted the warrant liability for changes in estimated

fair value until the earlier of the exercise, conversion, or expiration of the Redeemable Convertible Preferred Stock Warrants. In July

2024, the Redeemable Convertible Preferred Stock Warrants were amended to provide that if underlying Redeemable Convertible Preferred

Stock Warrants were out-of-the-money based on the initial public offering price in the IPO, the out-of-the-money Redeemable Convertible

Preferred Stock Warrants would convert into warrants to purchase common stock with an exercise price per share that reflected the Conversion

Ratio then in effect for the underlying Redeemable Convertible Preferred Stock. Accordingly, the Redeemable Convertible Preferred Stock

Warrants were remeasured upon the closing of the IPO and marked to market to their fair value before being reclassified to equity

The Redeemable Convertible Preferred

Stock Warrant Liability was valued using the Black-Scholes valuation model, which requires the use of highly subjective assumptions to

determine the appropriate fair value of each warrant, including:

Fair Value Option of Accounting for Related Party Convertible Notes

Payable

When financial instruments contain

various embedded derivatives which may require bifurcation and separate accounting of those derivatives apart from the entire host instrument,

if eligible, ASC 825, Financial Instruments (“ASC 825”) allows issuers to elect the fair value option (“FVO”)

of accounting for those instruments. The FVO may be elected on an instrument-by-instrument basis and is irrevocable unless a new election

date occurs. The FVO allows the issuer to account for the entire financial instrument at fair value with subsequent remeasurements of

that fair value recorded through the statements of operations at each reporting period until the conversion or payment of the Related

Party Convertible Notes Payable balance. A financial instrument is generally eligible for the FVO if, amongst other factors, no part of

the convertible, or contingently convertible, instrument is classified in stockholders’ equity.

Based on the eligibility assessment

discussed above, the Company concluded that its Related Party Convertible Notes Payable were eligible for the FVO and accordingly elected

to apply the FVO to its Related Party Convertible Notes Payable in accordance with ASC 825. Accordingly, the Related Party Convertible

Notes Payable were measured at fair value on their issuance dates and remeasured at estimated fair value at the end of each reporting

period with changes in fair value recognized as a component of other income (expense) in the consolidated statements of operations. The

primary reason for electing the fair value option was to address simplification and cost-benefit considerations that result from accounting

for hybrid financial instruments at fair value in their entirety versus bifurcation of the embedded derivatives from the debt hosts.

The estimated fair values of the

Related Party Convertible Notes Payable were determined using valuation models that incorporated assumptions and estimates. The Company

assessed these assumptions and estimates at each financial reporting period as additional information impacting the assumptions was obtained.

Assumptions in the models included but were not limited to equity value, volatility, time to a conversion event, risk-free rate and scenario

weightings. The fair value measurements of the Related Party Convertible Notes Payable were based on significant inputs that were not

observable in the market and represented a Level 3 measurement. The change in fair value related to accrued interest was also included

within the single line of change in fair value of Related Party Convertible Notes Payable in the consolidated statements of operations.

In addition, in certain circumstances,

the estimated fair value at issuance may be greater than the face value at issuance. The loss on issuance of the Related Party Convertible

Notes Payable recorded during year ended December 31, 2024 represented the difference between the estimated fair value of the Related

Party Convertible Notes Payable and the gross proceeds received on the issuance date based on the assumptions, including the proximity

in time to the anticipated IPO, the discount on conversion of the Related Party Convertible Notes Payable, and the increased probability-weighted

IPO scenario. In connection with the closing of the Company’s IPO on August 14, 2024, the Related Party Convertible Notes Payable

were converted into common stock and no amounts are outstanding as of December 31, 2024.

Off-Balance Sheet Arrangements

We did not have, during the periods

presented, and we do not currently have, any off-balance sheet arrangements, as defined in the rules and regulations of the SEC.

Recent Accounting Pronouncements

A description of recently issued

accounting standards that may potentially impact our financial position, results of operations, and cash flows is included in Note 2 to

our consolidated financial statements in this Report.

Emerging Growth Company Status and Smaller Reporting

Company Status

We are an emerging growth company,

as defined in the Jumpstart Our Business Startups Act of 2012 (the “JOBS Act”). The JOBS Act permits an emerging growth company

such as ours to take advantage of an extended transition period to comply with new or revised accounting standards. We have elected to

avail ourselves of such extended transition period, which means that when a standard is issued or revised and it has different application

dates for public or private companies, we can adopt the new or revised standard at the time private companies adopt the new or revised

standard and may do so until such time that we either (i) irrevocably elect to opt out of such extended transition period or (ii) no longer

qualify as an emerging growth company. We may choose to early adopt any new or revised accounting standards whenever such early adoption

is permitted for private companies. We will continue to remain an emerging growth company until the earliest of the following: (1) the

last day of the fiscal year following the fifth anniversary of the date of the completion of the IPO; (2) the last day of the fiscal year

in which our total annual gross revenue is equal to or more than $1.235 billion; (3) the date on which we have issued more than $1.0 billion

in nonconvertible debt during the previous three years; or (4) the date on which we are deemed to be a large accelerated filer under the

rules of the SEC.

We are also a smaller reporting

company as defined in the Securities Exchange Act of 1934, as amended (the “Exchange Act”). We may continue to be a smaller

reporting company even after we are no longer an emerging growth company. We may take advantage of certain of the scaled disclosures available

to smaller reporting companies and will be able to take advantage of these scaled disclosures for so long as our voting and non-voting

common stock held by non-affiliates is less than $250.0 million measured on the last business day of our second fiscal quarter, or our

annual revenue is less than $100.0 million during the most recently completed fiscal year and our voting and non-voting common stock held

by non-affiliates is less than $700.0 million measured on the last business day of our second fiscal quarter.

Item 7A. Quantitative and Qualitative Disclosures About Market Risk.

As a “smaller reporting

company” as defined in Rule 12b-2 of the Exchange Act, we are not required to provide the information called for by this item.

Item 8. Financial Statements and Supplementary Data.

Our consolidated financial statements,

together with the report of our independent registered public accounting firm, are included in this Annual Report on Form 10-K beginning

on page F-1.

Item 9. Changes in and Disagreements with Accountants on Accounting

and Financial Disclosure.

None.

Item 9A. Controls and Procedures.

Attestation Report of the Independent Registered Public Accounting

Firm

This Annual Report does not

include an attestation report of our independent registered public accounting firm due to an exemption provided by the JOBS Act for “emerging

growth companies.”

Evaluation of Disclosure Controls and Procedures

As required by Rule 13a-15(b) under

the Exchange Act, as of December 31, 2024, the end of the period to which this annual report relates, we have carried out an evaluation

of the effectiveness of the design and operation of our disclosure controls and procedures. This evaluation was carried out under the

supervision and with the participation of our management, including our President and Chief Executive Officer and our Chief Financial

Officer.

Disclosure controls and procedures

are controls and other procedures that are designed to ensure that information required to be disclosed in our reports filed or submitted

under the Exchange Act is recorded, processed, summarized and reported, within the time periods specified in the Securities and Exchange

Commission’s rules and forms. Disclosure controls and procedures include, without limitation, controls and procedures designed to

ensure that information required to be disclosed in our reports filed under the Exchange Act is accumulated and communicated to management,

including the President and Chief Executive Officer and the Chief Financial Officer as appropriate, to allow timely decisions regarding

required disclosure. Management recognizes that any controls and procedures, no matter how well designed and operated, can provide only

reasonable assurance of achieving their objectives, and management necessarily applies its judgment in evaluating the cost-benefit relationship

of possible controls and procedures. Based on the evaluation of our disclosure controls and procedures as of December 31, 2024, our management,

with the participation of our President and Chief Executive Officer and our Chief Financial Officer, concluded that, as of such date,

our disclosure controls and procedures were effective at a reasonable assurance level.

Material Weakness in Internal Control over Financial

Reporting

While management was not required

to conduct an assessment regarding internal control over financial reporting, we previously identified a material weakness as of December

31, 2023 as a result of an inadequate review and accrual of clinical trial related expenses that resulted in improper accrual of expenses.

A material weakness is a deficiency or combination of deficiencies in internal control over financial reporting such that there is a reasonable

possibility that a material misstatement of our consolidated financial statements would not be prevented or detected on a timely basis.

Changes in Internal Control over Financial Reporting

The Company has implemented remediation

procedures to address the material weakness in our internal control over financial reporting identified above, including the implementation

of additional processes and controls to properly review and monitor the accrual of clinical trial expenses at the end of each period during

the year ended December 31, 2024. The material weakness was remediated as of December 31, 2024.

Item 9B. Other Information.

Executive Compensation

On March 10, 2025, the Compensation

Committee of the Board recommended and the Board approved updates to executive officer compensation for 2025. The Compensation Committee

and the Board considered, among other factors, the compensation practices, trends and data from comparable companies and input from its

independent compensation consultant, with the adjustment resulting in compensation levels at approximately the 25th percentile of

the comparable company peer group.

Effective January 1, 2025,

the following updates to cash compensation amounts were approved, subject to the Company receiving at least $25 million in additional

funding from one or more financings and will only payable after receipt of at least $25 million in aggregate funding: (1) an increase

in Mr. Schmitt’s annual base salary to $570,000; and (2) an increase in Mr. Lytle’s annual base salary to $435,363.

In addition, the executive

officers will receive, with a grant date of April 1, 2025 and at an exercise price equal to the closing price of the Company’s common

stock as of such date, stock option grants consisting of (1) one-time grants to reflect a catch-up of salary that would have been paid

if such salary increases had taken effect on August 14, 2024, representing the IPO closing date, in the following amounts: Mr. Schmitt,

4,285 stock options; and Mr. Lytle, 1,900 stock options (the “Base Pay True Up Grants”); (2) one-time grants to reflect a

catch-up grant, since no annual grants were made in 2024, in the following amounts: Mr. Schmitt, 200,000 stock options; Mr. Lytle, 39,999

stock options; and Dr. Mazar, 110,346 stock options (the “Equity True Up Grants,” and together with the Base Pay True Up Grants,

the “Special Option Grants”); and (3) annual grants in the following amounts: Mr. Schmitt, 66,503 stock options; Mr. Lytle,

49,394 stock options; and Dr. Mazar, 51,046 stock options (the “Annual Option Grants”). The Special Option Grants will vest

as to 25% on August 14, 2025 with the remaining 75% vesting in equal monthly installments during the 36 months thereafter and the Annual

Grants will vest as to 25% on the first anniversary of the grant date with the remaining 75% vesting in equal monthly installments during

the 36 months following the first anniversary of the grant date.

The Board also approved (1)

an amendment to the employment agreement with Dr. Mazar to provide that the deadline by which the Company would need to complete a Sale

Transaction (as defined in the agreement) for him to receive an additional equity award be extended from March 31, 2024 to December 31,

2026 (to align with a similar provision in Mr. Schmitt’s agreement), and (2) an amendment to the employment agreement with Mr. Lytle

to provide for the issuance of an equity award in the event of a Sale Transaction (as defined in the agreement) completed on or before

December 31, 2026 (to align with similar provisions in each of Mr. Schmitt’s and Dr. Mazar’s agreements). See “Item

11. Executive Compensation – Employment Agreements” for additional information.

Rule 10b5-1 Trading Plans

During the quarter ended December

31, 2024, no director or officer of the Company adopted or terminated any Rule 10b5-1 trading arrangement or non-Rule 10b5-1 trading arrangement,

as each term is defined in Item 408(a) of Regulation S-K.

Item 9C. Disclosure Regarding Foreign Jurisdictions that Prevent Inspections.

Not applicable.

PART III

Item 10. Directors, Executive Officers and Corporate Governance.

Executive Officers and Directors

The table below lists the name,

age and position of each of our executive officers and director as February 28, 2025.

Name Age Position

Executive Officers:

Daniel M. Schmitt 63 President, Chief Executive Officer and Director

Andrew P. Mazar, Ph.D. 63 Chief Operating Officer

Paul Lytle 57 Chief Financial Officer

Non-Employee Directors:

Aaron G.L. Fletcher, Ph.D. 44 Chairperson and Director(1)

Jason Keyes 53 Director(3)

Source: SEC EDGAR (public domain) · 10-K for the period ended 2024-12-31, filed 2025-03-13 · accession 0001683168-25-001581

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